Hotspot

atpE-atpB

LSC · 48305-48324 · 20 bp

Back to Nanocnide

Pi 0.1
MarkerSeek score 76.20
Primer available yes
Label atpE-atpB

Feature Metadata

label_name
atpE-atpB
feature_type
igs
region
LSC
length_bp
20
variable_sites
2
pi
0.1
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE-atpB_p1 TGATTCGGAGTCAGTACACA TGATGAAGCTACTGCGAAGG 45.0 50.0 55.9 57.7 89 bp 1.0 62.8
atpE-atpB_p2 TGATTCGGAGTCAGTACACA GATGGTCTTCCTGAACAGGC 45.0 55.0 55.9 58.3 130 bp 1.0 62.6
atpE-atpB_p3 TCGGAGTCAGTACACAAAGA TGATGAAGCTACTGCGAAGG 45.0 50.0 55.9 57.7 85 bp 1.0 62.4
atpE-atpB_p4 TCGGAGTCAGTACACAAAGA GATGGTCTTCCTGAACAGGC 45.0 55.0 55.9 58.3 126 bp 1.0 62.1
atpE-atpB_p5 TGATTCGGAGTCAGTACACAA GATGGTCTTCCTGAACAGGC 42.9 55.0 56.7 58.3 130 bp 1.0 60.8