Hotspot

trnF-GAA-ndhJ

LSC · 44467-44866 · 400 bp

Back to Nanocnide

Pi 0.0596

MarkerSeek score 73.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 23
pi: 0.0596
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TTATGATGTAACACCGGGCG	55.0	50.0	58.0	58.1	780-893 bp	1.0	83.3
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	AGGATTGGCATTCGATTGCT	55.0	45.0	58.0	57.9	847-960 bp	1.0	83.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TTATGATGTAACACCGGGCG	50.0	50.0	58.1	58.1	744-857 bp	1.0	83.1
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GCACAGCCGTTTGTCTTTTT	55.0	45.0	58.0	58.1	945-1058 bp	1.0	82.9
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	AAGTTTTGTGTTGAACGGCG	55.0	45.0	58.0	58.1	1210-1323 bp	1.0	82.4