Hotspot

psbI-trnS-GCU

LSC · 6516-6614 · 99 bp

Back to Nanocnide

Pi 0.0659

MarkerSeek score 74.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 99
variable_sites: 6
pi: 0.0659
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CCTATCTAATGATCCGGGACG	TGGACTAAAGCGTCGGATTG	52.4	50.0	57.2	58.0	212-235 bp	1.0	70.7
psbI-trnS-GCU_p2	CCTATCTAATGATCCGGGACG	GTGGACTAAAGCGTCGGATT	52.4	50.0	57.2	58.0	213-236 bp	1.0	70.7
psbI-trnS-GCU_p3	CCTATCTAATGATCCGGGACG	TTTTTCGTACCGAGGGTTCG	52.4	50.0	57.2	58.2	175-198 bp	1.0	70.0
psbI-trnS-GCU_p4	CCTATCTAATGATCCGGGACG	GACTAAAGCGTCGGATTGCT	52.4	50.0	57.2	58.4	210-233 bp	1.0	67.9
psbI-trnS-GCU_p5	CCTATCTAATGATCCGGGACG	CTGAGTGGACTAAAGCGTCG	52.4	55.0	57.2	58.4	217-240 bp	1.0	67.6