Hotspot

psbA-trnK-UUU

LSC · 1287-1582 · 296 bp

Back to Nanocnide

Pi 0.0651
MarkerSeek score 74.40
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
296
variable_sites
17
pi
0.0651
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 TAAAGGCGGTTTTCAGTGCT ACTAGTTCCGGGTTCGAGTC 45.0 55.0 58.0 58.8 385-416 bp 1.0 80.6
psbA-trnK-UUU_p2 TAAAGGCGGTTTTCAGTGCT GACTAGTTCCGGGTTCGAGT 45.0 55.0 58.0 58.8 386-417 bp 1.0 80.6
psbA-trnK-UUU_p3 GTAAAGGCGGTTTTCAGTGC ACTAGTTCCGGGTTCGAGTC 50.0 55.0 58.0 58.8 386-417 bp 1.0 80.4
psbA-trnK-UUU_p4 GTAAAGGCGGTTTTCAGTGC GACTAGTTCCGGGTTCGAGT 50.0 55.0 58.0 58.8 387-418 bp 1.0 80.4
psbA-trnK-UUU_p5 TAAAGGCGGTTTTCAGTGCT ATCCGACTAGTTCCGGGTTC 45.0 55.0 58.0 58.9 390-421 bp 1.0 80.0