Hotspot

ycf3

LSC · 42001-43948 · 1948 bp

Back to Nannoglottis

Pi 0.00359

MarkerSeek score 57.90

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1948
variable_sites: 7
pi: 0.00359
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TATTTGACTGATGGCGCCAA	CGACTGAGCTAGGGGTCTAT	45.0	55.0	57.9	57.7	2700-2708 bp	1.0	77.0
ycf3_p2	TTATTTGACTGATGGCGCCA	CGACTGAGCTAGGGGTCTAT	45.0	55.0	57.9	57.7	2701-2709 bp	1.0	77.0
ycf3_p3	GATGGCGCCAACAAACAATT	CGACTGAGCTAGGGGTCTAT	45.0	55.0	58.2	57.7	2691-2699 bp	1.0	76.4
ycf3_p4	CGAGCGGCCATTAGCTATTA	CGACTGAGCTAGGGGTCTAT	50.0	55.0	57.6	57.7	2927-2937 bp	1.0	75.1
ycf3_p5	ACACATACAAAGAGACCCGC	GAATCCAATGCGGTTCCCAT	50.0	50.0	57.9	58.6	2918-2928 bp	1.0	74.7