Hotspot

ycf1

SSC · 108188-113359 · 5172 bp

Back to Nannoglottis

Pi 0.00866

MarkerSeek score 48.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5172
variable_sites: 44
pi: 0.00866
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCTTCCTTCTCCGAGCTCA	GCTTCTCTTCTTACGGAGCC	50.0	55.0	58.1	58.1	950-989 bp	1.0	83.9
ycf1_p2	TTCTTCCTTCTCCGAGCTCA	GGCTTCTCTTCTTACGGAGC	50.0	55.0	58.1	58.1	951-990 bp	1.0	83.9
ycf1_p3	CTAGCACTGGGTAGACCTCA	GCTTCTCTTCTTACGGAGCC	55.0	55.0	57.9	58.1	821-860 bp	1.0	83.5
ycf1_p4	CTAGCACTGGGTAGACCTCA	GGCTTCTCTTCTTACGGAGC	55.0	55.0	57.9	58.1	822-861 bp	1.0	83.5
ycf1_p5	TTCTTCCTTCTCCGAGCTCA	TCGTAATGGGCGATTCCATC	50.0	50.0	58.1	57.8	900-939 bp	1.0	83.0