Hotspot

trnL-UAG-rpl32

SSC · 122988-123894 · 907 bp

Back to Nannoglottis

Pi 0.00783
MarkerSeek score 60.70
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
907
variable_sites
7
pi
0.00783
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GAAAGGATATTGGGCGTCGT 50.0 50.0 58.0 58.1 1029-1036 bp 1.0 81.1
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GAAAGGATATTGGGCGTCGT 50.0 50.0 58.0 58.1 1030-1037 bp 1.0 81.1
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA GAAAGGATATTGGGCGTCGT 50.0 50.0 57.9 58.1 1039-1046 bp 1.0 80.1
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC GAAAGGATATTGGGCGTCGT 55.0 50.0 57.8 58.1 1038-1045 bp 1.0 79.6
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC GAAAGGATATTGGGCGTCGT 55.0 50.0 57.8 58.1 1037-1044 bp 1.0 79.6