Hotspot

ycf1-rps15

SSC · 113360-113762 · 403 bp

Back to Nannoglottis

Pi 0.00995

MarkerSeek score 56.00

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 403
variable_sites: 4
pi: 0.00995
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	GGGAGCCGTTTGAGTATGTT	AAGCCTCCGTATCTTGTTGG	50.0	50.0	57.9	57.6	558-559 bp	1.0	76.6
ycf1-rps15_p2	ATGGGAGCCGTTTGAGTATG	AAGCCTCCGTATCTTGTTGG	50.0	50.0	57.7	57.6	560-561 bp	1.0	74.8
ycf1-rps15_p3	GGGAGCCGTTTGAGTATGTT	GCCTCCGTATCTTGTTGGTG	50.0	55.0	57.9	58.6	556-557 bp	1.0	74.8
ycf1-rps15_p4	GGGAGCCGTTTGAGTATGTT	AGCCTCCGTATCTTGTTGGT	50.0	50.0	57.9	58.7	557-558 bp	1.0	74.1
ycf1-rps15_p5	ATGGGAGCCGTTTGAGTATG	GCCTCCGTATCTTGTTGGTG	50.0	55.0	57.7	58.6	558-559 bp	1.0	73.0