Hotspot

ycf4-cemA

LSC · 59985-60867 · 883 bp

Back to Nannoglottis

Pi 0.00577

MarkerSeek score 56.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 5
pi: 0.00577
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1049-1063 bp	1.0	80.7
ycf4-cemA_p2	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1013-1027 bp	1.0	80.3
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	981-995 bp	1.0	80.3
ycf4-cemA_p4	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	995-1009 bp	1.0	79.7
ycf4-cemA_p5	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	993-1007 bp	1.0	79.3