Hotspot

trnG-UCC-trnT-GGU

LSC · 30753-30950 · 198 bp

Back to Nannoglottis

Pi 0.0152

MarkerSeek score 61.20

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 3
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	AATCAAACCGAAAGACCCCT	ATTTGAACCGAGGGCTTACG	45.0	50.0	57.0	58.3	330-356 bp	1.0	70.8
trnG-UCC-trnT-GGU_p2	ATCAAACCGAAAGACCCCTT	ATTTGAACCGAGGGCTTACG	45.0	50.0	57.0	58.3	329-355 bp	1.0	70.8
trnG-UCC-trnT-GGU_p3	AATCAAACCGAAAGACCCCT	GGATTTGAACCGAGGGCTTA	45.0	50.0	57.0	57.6	332-358 bp	1.0	69.6
trnG-UCC-trnT-GGU_p4	ATCAAACCGAAAGACCCCTT	GGATTTGAACCGAGGGCTTA	45.0	50.0	57.0	57.6	331-357 bp	1.0	69.6
trnG-UCC-trnT-GGU_p5	GAATCAAACCGAAAGACCCCT	ATTTGAACCGAGGGCTTACG	47.6	50.0	58.2	58.3	331-357 bp	1.0	68.9