Hotspot

trnR-UCU-trnG-UCC

LSC · 29746-29953 · 208 bp

Back to Nannoglottis

Pi 0.0192

MarkerSeek score 63.60

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 4
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	47.6	50.0	56.9	58.0	288 bp	1.0	64.5
trnR-UCU-trnG-UCC_p2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	47.6	55.0	56.9	58.1	291 bp	1.0	64.2
trnR-UCU-trnG-UCC_p3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	45.5	50.0	57.9	58.0	294 bp	1.0	63.8
trnR-UCU-trnG-UCC_p4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	45.5	55.0	57.9	58.1	297 bp	1.0	63.5
trnR-UCU-trnG-UCC_p5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	47.6	55.0	56.9	57.7	292 bp	1.0	62.0