Hotspot

chlN

Genome · 94673-95998 · 1326 bp

Back to Nannochloropsis

Pi 0.0588

MarkerSeek score 79.50

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 1326
variable_sites: 145
pi: 0.0588
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	AGCGCTCCCATAAGCTAGTA	TCGCGAAAAAGCTAGGACTC	50.0	50.0	58.0	58.0	2013-2019 bp	0.75	75.6
chlN_p2	CACCGAACCAACCGATGTAT	TCGCGAAAAAGCTAGGACTC	50.0	50.0	58.0	58.0	2067-2073 bp	0.75	75.4
chlN_p3	GCGCTCCCATAAGCTAGTAC	TCGCGAAAAAGCTAGGACTC	55.0	50.0	57.9	58.0	2012-2018 bp	0.75	75.1
chlN_p4	CATTCACGTCCCATGTAGCA	TCGCGAAAAAGCTAGGACTC	50.0	50.0	58.0	58.0	2356-2362 bp	0.75	74.7
chlN_p5	CCCATTCACGTCCCATGTAG	TCGCGAAAAAGCTAGGACTC	55.0	50.0	58.0	58.0	2358-2364 bp	0.75	74.5