Hotspot

ycf49-psaL

Genome · 105732-106263 · 532 bp

Back to Nannochloropsis

Pi 0.0711

MarkerSeek score 79.70

Primer available yes

Label ycf49-psaL

Feature Metadata

label_name: ycf49-psaL
feature_type: igs
region: Genome
length_bp: 532
variable_sites: 65
pi: 0.0711
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf49-psaL_p1	ACTTTGTGCCCCAAATCCAT	GCAGAACGTAATGGGCCATA	45.0	50.0	58.0	58.0	1231-1234 bp	0.5	62.3
ycf49-psaL_p2	ACTTTGTGCCCCAAATCCAT	AAGGCTATACCAACTGCAGC	45.0	50.0	58.0	58.0	1519-1522 bp	0.5	62.3
ycf49-psaL_p3	ACTTTGTGCCCCAAATCCAT	TGGTGTTAGTCCTGGTCGAT	45.0	50.0	58.0	58.1	1148-1151 bp	0.5	62.2
ycf49-psaL_p4	GTTTTCGGACCGGACTATCC	GCAGAACGTAATGGGCCATA	55.0	50.0	58.1	58.0	1463 bp	0.25	49.1
ycf49-psaL_p5	GTTTTCGGACCGGACTATCC	AAGGCTATACCAACTGCAGC	55.0	50.0	58.1	58.0	1751 bp	0.25	49.1