Hotspot

psaI-psbJ

Genome · 103911-103965 · 55 bp

Back to Nannochloropsis

Pi 0.185

MarkerSeek score 79.70

Primer available yes

Label psaI-psbJ

Feature Metadata

label_name: psaI-psbJ
feature_type: igs
region: Genome
length_bp: 55
variable_sites: 16
pi: 0.185
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-psbJ_p1	GAAGGGGTTTTACCTGGTGG	GCTTGTAGCCTTTGTTGCAG	55.0	50.0	58.1	57.9	754-755 bp	0.75	77.2
psaI-psbJ_p2	GAAGGGGTTTTACCTGGTGG	TGTAGCCTTTGTTGCAGGTT	55.0	45.0	58.1	57.9	751-752 bp	0.75	77.1
psaI-psbJ_p3	GAAGGGGTTTTACCTGGTGG	TTGTAGCCTTTGTTGCAGGT	55.0	45.0	58.1	57.9	752-753 bp	0.75	77.1
psaI-psbJ_p4	GAAGGGGTTTTACCTGGTGG	ACGTTTCGTTGGTTAGCAGT	55.0	45.0	58.1	58.1	1088-1090 bp	0.75	75.2
psaI-psbJ_p5	GAAGGGGTTTTACCTGGTGG	GAACGCCCATTCTCAGACAT	55.0	50.0	58.1	58.0	1366-1368 bp	0.75	74.3