Hotspot

ycf54-secA

Genome · 55461-57755 · 2295 bp

Back to Nannochloropsis

Pi 0.103

MarkerSeek score 80.70

Primer available yes

Label ycf54-secA

Feature Metadata

label_name: ycf54-secA
feature_type: igs
region: Genome
length_bp: 2295
variable_sites: 422
pi: 0.103
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf54-secA_p1	CTCAGGCGAAGGAAGAATCC	GATGCAGGAAGAGTGGCTAC	55.0	55.0	58.1	58.1	2731-2783 bp	0.5	65.3
ycf54-secA_p2	CTCAGGCGAAGGAAGAATCC	GTACACCTTTGCCGCTAAGA	55.0	50.0	58.1	57.9	2812 bp	0.25	48.9
ycf54-secA_p3	CTCAGGCGAAGGAAGAATCC	TTGCCGCTAAGAGCTTGAAA	55.0	45.0	58.1	58.1	2804 bp	0.25	48.7
ycf54-secA_p4	CTCAGGCGAAGGAAGAATCC	TTTGCCGCTAAGAGCTTGAA	55.0	45.0	58.1	58.1	2805 bp	0.25	48.7
ycf54-secA_p5	CTCAGGCGAAGGAAGAATCC	CGAGCCCTCCAAGAATTTGA	55.0	50.0	58.1	57.9	2710 bp	0.25	48.7