Hotspot

P417_t002-P417_t003

Genome · 5895-6546 · 652 bp

Back to Nannochloropsis

Pi 0.0918
MarkerSeek score 86.30
Primer available yes
Label P417_t002-P417_t003

Feature Metadata

label_name
P417_t002-P417_t003
feature_type
igs
region
Genome
length_bp
652
variable_sites
109
pi
0.0918
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
P417_t002-P417_t003_p1 GCTACCAATTTCGCCACATC TGGAGACCTACGTTCTACCA 50.0 50.0 57.5 57.1 707-732 bp 1.0 79.7
P417_t002-P417_t003_p2 TACCAATTTCGCCACATCCG TGGAGACCTACGTTCTACCA 50.0 50.0 58.6 57.1 705-730 bp 1.0 78.5
P417_t002-P417_t003_p3 GCTACCAATTTCGCCACATC TGGAGACCTACGTTCTACCAA 50.0 47.6 57.5 57.8 707-732 bp 1.0 77.5
P417_t002-P417_t003_p4 GCTACCAATTTCGCCACATC TTGGAGACCTACGTTCTACCA 50.0 47.6 57.5 57.8 708-733 bp 1.0 77.5
P417_t002-P417_t003_p5 GGCTACCAATTTCGCCACAT TGGAGACCTACGTTCTACCA 50.0 50.0 58.9 57.1 708-733 bp 1.0 76.3