Hotspot

rps12

Genome · 16937-121177 · 104241 bp

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Pi 0.000204

MarkerSeek score 50.50

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: Genome
length_bp: 104241
variable_sites: 15
pi: 0.000204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	AAAATTAGGATGATGCGTCA	AGAGCATATCTCCAGACACCT	35.0	47.6	53.0	57.7	223 bp	1.0	40.7
rps12_p2	AAATTAGGATGATGCGTCAA	AGAGCATATCTCCAGACACCT	35.0	47.6	53.0	57.7	222 bp	1.0	40.7
rps12_p3	AAAATTAGGATGATGCGTCAA	AGAGCATATCTCCAGACACCT	33.3	47.6	53.9	57.7	223 bp	1.0	40.7
rps12_p4	ACTACTGAAACTGACAAAGTCT	AGAGCATATCTCCAGACACCT	36.4	47.6	55.0	57.7	285 bp	1.0	40.6
rps12_p5	ACTACTGAAACTGACAAAGTCTT	AGAGCATATCTCCAGACACCT	34.8	47.6	55.8	57.7	285 bp	1.0	40.6