Hotspot

cemA

Genome · 25696-26385 · 690 bp

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Pi 0.00145

MarkerSeek score 59.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 690
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	AGGAAGAATACGAGTAGAAAGA	TGACAAATGAGATGAAGCTGAGT	36.4	39.1	53.8	58.1	832 bp	1.0	40.1
cemA_p2	AGGAAGAATACGAGTAGAAAGAA	TGACAAATGAGATGAAGCTGAGT	34.8	39.1	54.6	58.1	832 bp	1.0	40.1
cemA_p3	AGGAAGAATACGAGTAGAAAGAAA	TGACAAATGAGATGAAGCTGAGT	33.3	39.1	55.3	58.1	832 bp	1.0	40.1
cemA_p4	AGGAAGAATACGAGTAGAAAGA	TGACAAATGAGATGAAGCTGAG	36.4	40.9	53.8	56.4	832 bp	1.0	40.1
cemA_p5	AGGAAGAATACGAGTAGAAAGA	GACAAATGAGATGAAGCTGAGT	36.4	40.9	53.8	56.4	831 bp	1.0	40.1