Hotspot

atpF

Genome · 37923-39184 · 1262 bp

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Pi 0.000792

MarkerSeek score 59.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 1262
variable_sites: 1
pi: 0.000792
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TGCACGAATGGTTACCATGA	CCATAAGAGGAGATGCGATCA	45.0	47.6	57.5	57.1	1377 bp	1.0	60.6
atpF_p2	TGCACGAATGGTTACCATGA	TCCATAAGAGGAGATGCGATC	45.0	47.6	57.5	56.8	1378 bp	1.0	58.4
atpF_p3	ATCTGCACGAATGGTTACCA	CCATAAGAGGAGATGCGATCA	45.0	47.6	57.2	57.1	1380 bp	1.0	58.2
atpF_p4	CTGCACGAATGGTTACCATG	CCATAAGAGGAGATGCGATCA	50.0	47.6	57.2	57.1	1378 bp	1.0	57.8
atpF_p5	TGCACGAATGGTTACCATGA	CCATAAGAGGAGATGCGATCAT	45.0	45.5	57.5	57.5	1377 bp	1.0	56.5