Hotspot

trnF-GAA-ndhJ

Genome · 76367-77117 · 751 bp

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Pi 0.00266

MarkerSeek score 60.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 751
variable_sites: 2
pi: 0.00266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TTGGATAGGATGGCCTTTGC	55.0	50.0	58.0	57.9	891 bp	1.0	79.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	855 bp	1.0	79.2
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	TTGGATAGGATGGCCTTTGC	50.0	50.0	57.9	57.9	900 bp	1.0	78.9
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	TTGGATAGGATGGCCTTTGC	55.0	50.0	57.9	57.9	879 bp	1.0	78.5
trnF-GAA-ndhJ_p5	AGTTCAAATCTGGTTCCCGG	TTGGATAGGATGGCCTTTGC	50.0	50.0	57.8	57.9	843 bp	1.0	78.0