Hotspot

atpH-atpI

Genome · 39904-41326 · 1423 bp

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Pi 0.000703

MarkerSeek score 59.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 1423
variable_sites: 1
pi: 0.000703
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGCAATCCCTTCTACAGCTT	GCTGCGGCTTATATAGGTGA	50.0	50.0	58.0	57.3	1585 bp	1.0	73.9
atpH-atpI_p2	CGCAATCCCTTCTACAGCTT	ACAAGCGGTATTCAAGCTCT	50.0	45.0	58.0	57.2	1621 bp	1.0	73.6
atpH-atpI_p3	CGCAATCCCTTCTACAGCTT	GGTGAATCCATGGAAGGTCA	50.0	50.0	58.0	57.2	1570 bp	1.0	73.4
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	GCTGCGGCTTATATAGGTGA	45.0	50.0	58.1	57.3	1495 bp	1.0	73.3
atpH-atpI_p5	AACAGAAGCGGCAGAAATCA	ACAAGCGGTATTCAAGCTCT	45.0	45.0	58.1	57.2	1531 bp	1.0	73.1