Hotspot

petL-psbE

Genome · 21154-22649 · 1496 bp

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Pi 0.000668

MarkerSeek score 59.10

Primer available yes

Label petL-psbE

Feature Metadata

label_name: petL-psbE
feature_type: igs
region: Genome
length_bp: 1496
variable_sites: 1
pi: 0.000668
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-psbE_p1	AACCGCTAGTAGAAAACCGA	TGACAAATAACCAACCCGCA	45.0	45.0	56.6	58.0	1653 bp	1.0	68.7
petL-psbE_p2	ACCGCTAGTAGAAAACCGAA	TGACAAATAACCAACCCGCA	45.0	45.0	56.6	58.0	1652 bp	1.0	68.7
petL-psbE_p3	AACCGCTAGTAGAAAACCGA	CAACCCGCAATGAATAGGGA	45.0	50.0	56.6	57.9	1642 bp	1.0	68.4
petL-psbE_p4	ACCGCTAGTAGAAAACCGAA	CAACCCGCAATGAATAGGGA	45.0	50.0	56.6	57.9	1641 bp	1.0	68.4
petL-psbE_p5	ACCGCTAGTAGAAAACCGAA	AGAACGTTCTCCTGTGCTTC	45.0	50.0	56.6	57.8	1564 bp	1.0	67.6