Hotspot

psbB-rps12

Genome · 14617-16936 · 2320 bp

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Pi 0

MarkerSeek score 52.20

Primer available yes

Label psbB-rps12

Feature Metadata

label_name: psbB-rps12
feature_type: igs
region: Genome
length_bp: 2320
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-rps12_p1	TGGACAGAAAGTAAACGGCC	AGAGCGGGAGATTTTGTGAC	50.0	50.0	58.1	57.9	2458-2459 bp	1.0	78.3
psbB-rps12_p2	AAACGGCCGGGATCATTTAA	AGAGCGGGAGATTTTGTGAC	45.0	50.0	57.9	57.9	2446-2447 bp	1.0	78.2
psbB-rps12_p3	TGGACAGAAAGTAAACGGCC	ACCCTAGTACAGGTTCCTCG	50.0	55.0	58.1	57.9	2497-2498 bp	1.0	78.0
psbB-rps12_p4	AAACGGCCGGGATCATTTAA	ACCCTAGTACAGGTTCCTCG	45.0	55.0	57.9	57.9	2485-2486 bp	1.0	77.9
psbB-rps12_p5	TGGACAGAAAGTAAACGGCC	GAGCGGGAGATTTTGTGACA	50.0	50.0	58.1	58.2	2457-2458 bp	1.0	77.6