Hotspot

rps16

LSC · 5004-6680 · 1677 bp

Pi 0.124

MarkerSeek score 83.50

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1677
variable_sites: 197
pi: 0.124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGTTTAGGGTTGGATTGGCA	GTAATAAATGCGATGCGGCC	50.0	50.0	58.1	58.0	2576 bp	0.5	59.2
rps16_p2	GGTTTAGGGTTGGATTGGCA	ACACAATCGCGACATACACA	50.0	45.0	58.1	57.9	2368 bp	0.5	58.9
rps16_p3	CCAACCGGACAGAAATGGAT	GTAATAAATGCGATGCGGCC	50.0	50.0	57.9	58.0	2703 bp	0.5	58.7
rps16_p4	AGTCAAAAACCAACCGGACA	GTAATAAATGCGATGCGGCC	45.0	50.0	57.9	58.0	2712 bp	0.5	58.7
rps16_p5	CCAACCGGACAGAAATGGAT	ACACAATCGCGACATACACA	50.0	45.0	57.9	57.9	2495 bp	0.5	58.4