Hotspot

rps18-rpl20

LSC · 71901-72481 · 581 bp

Back to Najas

Pi 0.0954
MarkerSeek score 84.20
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
581
variable_sites
52
pi
0.0954
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 TGACCTTGAAACAACAACGA AGGTATATCATATACAGAATGCT 40.0 30.4 55.8 52.1 687-710 bp 1.0 50.0
rps18-rpl20_p2 TGACCTTGAAACAACAACGA AGGTATATCATATACAGAATGCTT 40.0 29.2 55.8 52.9 687-710 bp 1.0 50.0
rps18-rpl20_p3 TGACCTTGAAACAACAACGA AAGGTATATCATATACAGAATGCT 40.0 29.2 55.8 52.9 688-711 bp 1.0 49.9
rps18-rpl20_p4 TTGACCTTGAAACAACAACGA AGGTATATCATATACAGAATGCT 38.1 30.4 56.5 52.1 688-711 bp 1.0 49.9
rps18-rpl20_p5 TGACCTTGAAACAACAACGA AAGGTATATCATATACAGAATGCTT 40.0 28.0 55.8 53.7 688-711 bp 1.0 49.9