Hotspot

tRNA-Thr(UGU)-tRNA-Leu (AAA)

LSC · 50058-50889 · 832 bp

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Pi 0.144
MarkerSeek score 84.50
Primer available yes
Label tRNA-Thr(UGU)-tRNA-Leu (AAA)

Feature Metadata

label_name
tRNA-Thr(UGU)-tRNA-Leu (AAA)
feature_type
igs
region
LSC
length_bp
832
variable_sites
114
pi
0.144
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Thr(UGU)-tRNA-Leu (AAA)_p1 AAAACCTGTCCTGTTGGTCC CATACCAACGGCTCAATCCA 50.0 50.0 57.9 58.0 1607 bp 0.5 59.2
tRNA-Thr(UGU)-tRNA-Leu (AAA)_p2 AGCGGCTTGACATTTGAAGA CATACCAACGGCTCAATCCA 45.0 50.0 58.1 58.0 1054 bp 0.5 58.9
tRNA-Thr(UGU)-tRNA-Leu (AAA)_p3 AAAACCTGTCCTGTTGGTCC ACCAACGGCTCAATCCAATT 50.0 45.0 57.9 58.1 1604 bp 0.5 58.8
tRNA-Thr(UGU)-tRNA-Leu (AAA)_p4 CTAAAGCGGAACAGTTGGGT CATACCAACGGCTCAATCCA 50.0 50.0 58.1 58.0 1475 bp 0.5 58.8
tRNA-Thr(UGU)-tRNA-Leu (AAA)_p5 GCGATGCTCTAACCTCTGAG CATACCAACGGCTCAATCCA 55.0 50.0 57.9 58.0 920 bp 0.5 58.6