Hotspot

psbZ-tRNA-Gly(GCC)

LSC · 39631-40264 · 634 bp

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Pi 0.112
MarkerSeek score 90.50
Primer available yes
Label psbZ-tRNA-Gly(GCC)

Feature Metadata

label_name
psbZ-tRNA-Gly(GCC)
feature_type
igs
region
LSC
length_bp
634
variable_sites
66
pi
0.112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
90.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-tRNA-Gly(GCC)_p1 TGCTTCTTCTGATGGTTGGT AATCGAACCCGCATCTTCTC 45.0 50.0 57.0 58.1 788-801 bp 1.0 82.5
psbZ-tRNA-Gly(GCC)_p2 TGCTTCTTCTGATGGTTGGT GAATCGAACCCGCATCTTCT 45.0 50.0 57.0 58.1 789-802 bp 1.0 82.5
psbZ-tRNA-Gly(GCC)_p3 GCTTCTTCTGATGGTTGGTCA AATCGAACCCGCATCTTCTC 47.6 50.0 58.2 58.1 787-800 bp 1.0 80.4
psbZ-tRNA-Gly(GCC)_p4 TGCTTCTTCTGATGGTTGGT CGTGATGATATTGGGCGGAT 45.0 50.0 57.0 57.9 827 bp 0.5 51.5
psbZ-tRNA-Gly(GCC)_p5 TGCTTCTTCTGATGGTTGGT TTCGTGATGATATTGGGCGG 45.0 50.0 57.0 58.1 829 bp 0.5 51.4