Hotspot

tRNA-Gln(UUG)-psbK

LSC · 8374-9190 · 817 bp

Back to Najas

Pi 0.0833
MarkerSeek score 81.90
Primer available yes
Label tRNA-Gln(UUG)-psbK

Feature Metadata

label_name
tRNA-Gln(UUG)-psbK
feature_type
igs
region
LSC
length_bp
817
variable_sites
62
pi
0.0833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Gln(UUG)-psbK_p1 GACCAAAACCCGTTGCCTTA ACAGATACAGATGAAACTAAAGA 50.0 30.4 58.7 52.9 851-888 bp 1.0 49.7
tRNA-Gln(UUG)-psbK_p2 TAACAGGACCAAAACCCGTT ACAGATACAGATGAAACTAAAGA 45.0 30.4 57.3 52.9 857-894 bp 1.0 49.7
tRNA-Gln(UUG)-psbK_p3 AACAGGACCAAAACCCGTTG ACAGATACAGATGAAACTAAAGA 50.0 30.4 58.9 52.9 856-893 bp 1.0 49.7
tRNA-Gln(UUG)-psbK_p4 ATAACAGGACCAAAACCCGT ACAGATACAGATGAAACTAAAGA 45.0 30.4 57.0 52.9 858-895 bp 1.0 49.7
tRNA-Gln(UUG)-psbK_p5 AAAACCCGTTGCCTTACCAC ACAGATACAGATGAAACTAAAGA 50.0 30.4 59.0 52.9 847-884 bp 1.0 49.7