Hotspot

clpP-ycf1

Genome · 84781-85412 · 632 bp

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Pi 0.00796
MarkerSeek score 63.90
Primer available yes
Label clpP-ycf1

Feature Metadata

label_name
clpP-ycf1
feature_type
igs
region
Genome
length_bp
632
variable_sites
5
pi
0.00796
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-ycf1_p1 ATGAGCAGGATTTTGCACGA TAACCACGCGTATACCAACG 45.0 50.0 58.2 58.1 704-713 bp 1.0 78.9
clpP-ycf1_p2 AATTGGCAAGAGAAGGACGG TAACCACGCGTATACCAACG 50.0 50.0 58.2 58.1 762-771 bp 1.0 78.8
clpP-ycf1_p3 ATGAGCAGGATTTTGCACGA CAGCGGTAATGTGGAAAGGA 45.0 50.0 58.2 57.9 767-776 bp 1.0 78.7
clpP-ycf1_p4 AATTGGCAAGAGAAGGACGG CAGCGGTAATGTGGAAAGGA 50.0 50.0 58.2 57.9 825-834 bp 1.0 78.6
clpP-ycf1_p5 AATTGGCAAGAGAAGGACGG TCAGCGGTAATGTGGAAAGG 50.0 50.0 58.2 57.9 826-835 bp 1.0 78.6