Hotspot

psbB-clpP

Genome · 83453-84108 · 656 bp

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Pi 0.00766
MarkerSeek score 63.60
Primer available yes
Label psbB-clpP

Feature Metadata

label_name
psbB-clpP
feature_type
igs
region
Genome
length_bp
656
variable_sites
5
pi
0.00766
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-clpP_p1 AGCCCAACCGGAAACTAAAG TGAGGTACTTTTGGAACGCC 50.0 50.0 58.1 58.1 785-787 bp 1.0 79.2
psbB-clpP_p2 CCCAACCGGAAACTAAAGCT TGAGGTACTTTTGGAACGCC 50.0 50.0 58.1 58.1 783-785 bp 1.0 79.2
psbB-clpP_p3 GGTATGGACGCGATACCAAG TGAGGTACTTTTGGAACGCC 55.0 50.0 58.2 58.1 713-715 bp 1.0 78.2
psbB-clpP_p4 GTATGGACGCGATACCAAGG TGAGGTACTTTTGGAACGCC 55.0 50.0 58.2 58.1 712-714 bp 1.0 78.2
psbB-clpP_p5 CGATACCAAGGCAAACCCAT TGAGGTACTTTTGGAACGCC 50.0 50.0 58.2 58.1 703-705 bp 1.0 78.1