Hotspot

rpl2-rps19

Genome · 71789-71844 · 56 bp

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Pi 0.0179
MarkerSeek score 62.80
Primer available yes
Label rpl2-rps19

Feature Metadata

label_name
rpl2-rps19
feature_type
igs
region
Genome
length_bp
56
variable_sites
1
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rps19_p1 GTGATGCTTCGATCCTTCGT CCGGGACCAGGTCACTATTA 50.0 55.0 58.1 58.2 199 bp 1.0 78.1
rpl2-rps19_p2 CTATAGCGCGCTTGGAAGAA CCGGGACCAGGTCACTATTA 50.0 55.0 58.1 58.2 243 bp 1.0 77.4
rpl2-rps19_p3 TGATGCTTCGATCCTTCGTC CCGGGACCAGGTCACTATTA 50.0 55.0 57.8 58.2 198 bp 1.0 77.0
rpl2-rps19_p4 AGTGATGCTTCGATCCTTCG CCGGGACCAGGTCACTATTA 50.0 55.0 57.8 58.2 200 bp 1.0 76.9
rpl2-rps19_p5 CGATCCTTCGTCGACGTAAG CCGGGACCAGGTCACTATTA 55.0 55.0 58.3 58.2 190 bp 1.0 76.5