Hotspot

psbL-psbF

Genome · 64536-64569 · 34 bp

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Pi 0.0294
MarkerSeek score 69.40
Primer available yes
Label psbL-psbF

Feature Metadata

label_name
psbL-psbF
feature_type
igs
region
Genome
length_bp
34
variable_sites
1
pi
0.0294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbL-psbF_p1 TAACAACCCCCAGTAGAGGC AATGCAGTTCATCCAGCGAT 55.0 45.0 58.7 58.0 125 bp 1.0 74.5
psbL-psbF_p2 TAACAACCCCCAGTAGAGGC CGTTCACGGACTAGCTGTAC 55.0 55.0 58.7 58.1 176 bp 1.0 73.9
psbL-psbF_p3 TAACAACCCCCAGTAGAGGC AGTTAGATGGTTGGCCGTTC 55.0 50.0 58.7 57.9 191 bp 1.0 73.8
psbL-psbF_p4 TAACAACCCCCAGTAGAGGC CAGTTAGATGGTTGGCCGTT 55.0 50.0 58.7 58.2 192 bp 1.0 73.2
psbL-psbF_p5 TAACAACCCCCAGTAGAGGC TTAGATGGTTGGCCGTTCAC 55.0 50.0 58.7 58.2 189 bp 1.0 73.2