Hotspot

trnF-GAA-ndhJ

Genome · 47915-48426 · 512 bp

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Pi 0.0215
MarkerSeek score 66.60
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
Genome
length_bp
512
variable_sites
11
pi
0.0215
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 AGGACTGAAAATCCTCGTGC TTGGCCTTTACGCAAAGACT 50.0 45.0 57.9 58.0 618-619 bp 1.0 80.6
trnF-GAA-ndhJ_p2 AGGACTGAAAATCCTCGTGC GTTGGCCTTTACGCAAAGAC 50.0 50.0 57.9 58.0 619-620 bp 1.0 80.4
trnF-GAA-ndhJ_p3 AGGACTGAAAATCCTCGTGC TGAGAGTTGGATTGGTTGGC 50.0 50.0 57.9 58.1 633-634 bp 1.0 80.0
trnF-GAA-ndhJ_p4 AGGACTGAAAATCCTCGTGC AGTTGGATTGGTTGGCCTTT 50.0 45.0 57.9 57.9 629-630 bp 1.0 79.9
trnF-GAA-ndhJ_p5 GACTGAAAATCCTCGTGCCA TTGGCCTTTACGCAAAGACT 50.0 45.0 58.2 58.0 616-617 bp 1.0 79.8