Hotspot

trnT-UGU-trnL-UAA

Genome · 46555-46957 · 403 bp

Back to Nageia

Pi 0.0175
MarkerSeek score 56.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
Genome
length_bp
403
variable_sites
7
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 CAAGTGCGATGCTCTAACCT CGTTTCCATACCAAGGCTCA 50.0 50.0 58.0 57.9 498-501 bp 1.0 80.6
trnT-UGU-trnL-UAA_p2 CAAGTGCGATGCTCTAACCT ACGTTTCCATACCAAGGCTC 50.0 50.0 58.0 57.9 499-502 bp 1.0 80.6
trnT-UGU-trnL-UAA_p3 CAAGTGCGATGCTCTAACCT ATTCGATCAAGTCCGTAGCG 50.0 50.0 58.0 57.9 478-481 bp 1.0 80.6
trnT-UGU-trnL-UAA_p4 CAAGTGCGATGCTCTAACCT CCGTAGCGTCTACCAATTCC 50.0 55.0 58.0 58.2 466-469 bp 1.0 80.4
trnT-UGU-trnL-UAA_p5 CAAGTGCGATGCTCTAACCT GGGTTCCCTGGATTTGGAAG 50.0 55.0 58.0 58.1 531-534 bp 1.0 80.2