Hotspot

psbM-trnD-GUC

Genome · 29120-30463 · 1344 bp

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Pi 0.0158
MarkerSeek score 58.50
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
Genome
length_bp
1344
variable_sites
21
pi
0.0158
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TGAACAATGCAACAGCAAGA TTAGAGTACCGCCCTGTCAA 40.0 50.0 56.5 58.1 1430-1442 bp 1.0 69.0
psbM-trnD-GUC_p2 TGAACAATGCAACAGCAAGA ATTGGTTAGAGTACCGCCCT 40.0 50.0 56.5 58.1 1435-1447 bp 1.0 68.6
psbM-trnD-GUC_p3 TGAACAATGCAACAGCAAGAA TTAGAGTACCGCCCTGTCAA 38.1 50.0 57.2 58.1 1430-1442 bp 1.0 66.9
psbM-trnD-GUC_p4 TGAACAATGCAACAGCAAGA TTACTGCGGATTAGTTGGCG 40.0 50.0 56.5 58.4 1480-1492 bp 1.0 66.7
psbM-trnD-GUC_p5 TGAACAATGCAACAGCAAGAA ATTGGTTAGAGTACCGCCCT 38.1 50.0 57.2 58.1 1435-1447 bp 1.0 66.5