Hotspot

rpl16

LSC · 83123-84551 · 1429 bp

Back to Myrsine

Pi 0.0102

MarkerSeek score 53.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1429
variable_sites: 27
pi: 0.0102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	ATTGCACGTGTCGAATGGAT	50.0	45.0	58.0	58.3	1912-1932 bp	1.0	80.5
rpl16_p2	ACACATCAATTCTCGAGCCC	CACGTGTCGAATGGATCAGA	50.0	50.0	58.0	57.7	1908-1928 bp	1.0	80.4
rpl16_p3	TAAATGCCTCAATTCCCGGG	ATTGCACGTGTCGAATGGAT	50.0	45.0	57.9	58.3	2176-2196 bp	1.0	80.0
rpl16_p4	TAAATGCCTCAATTCCCGGG	CACGTGTCGAATGGATCAGA	50.0	50.0	57.9	57.7	2172-2192 bp	1.0	79.9
rpl16_p5	ACACATCAATTCTCGAGCCC	AAATTGCACGTGTCGAATGG	50.0	45.0	58.0	57.7	1914-1934 bp	1.0	79.9