Hotspot

ccsA-ndhD

SSC · 117303-117574 · 272 bp

Back to Myrsine

Pi 0.0482

MarkerSeek score 74.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 272
variable_sites: 22
pi: 0.0482
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTGGACCGTATTCGCGATTT	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	573-600 bp	1.0	87.4
ccsA-ndhD_p2	TTGGACCGTATTCGCGATTT	TATCTTGACGAAATGGGCGG	45.0	50.0	58.0	58.1	867-894 bp	1.0	85.6
ccsA-ndhD_p3	TTGGACCGTATTCGCGATTT	TGACGAAATGGGCGGAATAG	45.0	50.0	58.0	58.1	862-889 bp	1.0	85.6
ccsA-ndhD_p4	TTGGACCGTATTCGCGATTT	TGGTGCTGCACTCTTTTTCT	45.0	45.0	58.0	57.9	922-949 bp	1.0	85.2
ccsA-ndhD_p5	ACCTGCTCTCCAATCTGAGT	GGACCGCGAGAGTTATTTGT	50.0	50.0	58.1	58.0	981-1008 bp	1.0	84.8