Hotspot

ndhF-rpl32

SSC · 114331-115166 · 836 bp

Back to Myrsine

Pi 0.0212

MarkerSeek score 63.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 836
variable_sites: 33
pi: 0.0212
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACAAACGCCTTTTCACAAGC	GCCCGGTGGAAAGAGATTTA	45.0	50.0	58.1	57.6	1340-1595 bp	1.0	82.2
ndhF-rpl32_p2	CAAACGCCTTTTCACAAGCA	GCCCGGTGGAAAGAGATTTA	45.0	50.0	58.1	57.6	1339-1594 bp	1.0	82.2
ndhF-rpl32_p3	AAAAGCCCACATACGACGAA	GCCCGGTGGAAAGAGATTTA	45.0	50.0	57.8	57.6	927-1182 bp	1.0	82.1
ndhF-rpl32_p4	ACAAACGCCTTTTCACAAGC	AAACTTTTTGACTGCCCGGT	45.0	45.0	58.1	58.5	1353-1608 bp	1.0	81.4
ndhF-rpl32_p5	CAAACGCCTTTTCACAAGCA	AAACTTTTTGACTGCCCGGT	45.0	45.0	58.1	58.5	1352-1607 bp	1.0	81.3