Hotspot

atpB-rbcL

LSC · 55680-56440 · 761 bp

Back to Myrsine

Pi 0.0484

MarkerSeek score 77.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 761
variable_sites: 73
pi: 0.0484
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CCGACTGGAACACTTAGAGC	CATGTACCTGCAGTAGCGTT	55.0	50.0	58.0	58.0	1808-1809 bp	0.75	71.0
atpB-rbcL_p2	AATTCGTCCGAGAGTTGCTC	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1314-1315 bp	0.75	70.9
atpB-rbcL_p3	CCGACTGGAACACTTAGAGC	GTAAGTCCATCGGTCCACAC	55.0	55.0	58.0	58.0	1292-1293 bp	0.75	70.9
atpB-rbcL_p4	AATTCGTCCGAGAGTTGCTC	CATGTACCTGCAGTAGCGTT	50.0	50.0	58.0	58.0	1830-1831 bp	0.75	70.9
atpB-rbcL_p5	GAACACTTAGAGCAGCTCCC	GTAAGTCCATCGGTCCACAC	55.0	55.0	58.0	58.0	1285-1286 bp	0.75	70.8