Hotspot

trnM-CAU-atpE

LSC · 53568-53784 · 217 bp

Back to Myrsine

Pi 0.033

MarkerSeek score 71.20

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 217
variable_sites: 12
pi: 0.033
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCAGGAGTCATTGGTTCAA	CGAGTCGAAGCTATCACTGG	50.0	55.0	58.1	57.9	275-282 bp	1.0	83.8
trnM-CAU-atpE_p2	TTGCTTTCATACGGCAGGAG	CGAGTCGAAGCTATCACTGG	50.0	55.0	58.3	57.9	287-294 bp	1.0	82.2
trnM-CAU-atpE_p3	CATACGGCAGGAGTCATTGG	CGAGTCGAAGCTATCACTGG	55.0	55.0	58.4	57.9	280-287 bp	1.0	81.1
trnM-CAU-atpE_p4	ATTGCTTTCATACGGCAGGA	CGAGTCGAAGCTATCACTGG	45.0	55.0	57.6	57.9	288-295 bp	1.0	80.9
trnM-CAU-atpE_p5	GGCAGGAGTCATTGGTTCAA	GCGAGTCGAAGCTATCACTG	50.0	55.0	58.1	58.5	276-283 bp	1.0	80.5