Hotspot

trnT-GGU-psbD

LSC · 32940-34281 · 1342 bp

Back to Myrsine

Pi 0.0129

MarkerSeek score 63.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1342
variable_sites: 31
pi: 0.0129
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1476-1515 bp	1.0	80.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1479-1518 bp	1.0	80.7
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1475-1514 bp	1.0	79.7
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1478-1517 bp	1.0	79.5
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	GCAATAAACCGGACCAACCT	55.0	50.0	58.1	58.5	1496-1535 bp	1.0	79.1