Hotspot

petN-psbM

LSC · 29339-30319 · 981 bp

Back to Myrsine

Pi 0.0133

MarkerSeek score 62.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 981
variable_sites: 26
pi: 0.0133
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TCTCGTGCCTACTGCTTTTC	50.0	50.0	58.2	57.9	1134-1166 bp	1.0	80.2
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	CTCGTGCCTACTGCTTTTCT	50.0	50.0	58.2	57.9	1133-1165 bp	1.0	80.1
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ACTGCATTGTTCGTTCTCGT	50.0	45.0	58.2	58.1	1148-1180 bp	1.0	79.7
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	GTTCTCGTGCCTACTGCTTT	50.0	50.0	58.2	58.2	1136-1168 bp	1.0	79.2
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	CTGCATTGTTCGTTCTCGTG	50.0	50.0	58.2	57.8	1147-1179 bp	1.0	79.1