Hotspot

trnS-GCU-trnS-CGA

LSC · 9166-9885 · 720 bp

Back to Myrsine

Pi 0.0199

MarkerSeek score 65.70

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 26
pi: 0.0199
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CTTTCGTCCACTCAGCCATC	ACCACTAAACTATACCCGCT	55.0	45.0	58.6	55.6	744-781 bp	1.0	62.7
trnS-GCU-trnS-CGA_p2	TTTCGTCCACTCAGCCATCT	ACCACTAAACTATACCCGCT	50.0	45.0	59.0	55.6	743-780 bp	1.0	59.6
trnS-GCU-trnS-CGA_p3	TCCACTCAGCCATCTCTCC	ACCACTAAACTATACCCGCT	57.9	45.0	58.1	55.6	738-775 bp	1.0	59.1
trnS-GCU-trnS-CGA_p4	ATTAGCAATCCGCCGCTTT	ACCACTAAACTATACCCGCT	47.4	45.0	58.2	55.6	759-796 bp	1.0	58.0
trnS-GCU-trnS-CGA_p5	TTCGTCCACTCAGCCATCT	ACCACTAAACTATACCCGCT	52.6	45.0	58.3	55.6	742-779 bp	1.0	57.2