Hotspot

rps16-trnQ-UUG

LSC · 6150-7904 · 1755 bp

Back to Myrsine

Pi 0.0184

MarkerSeek score 64.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1755
variable_sites: 61
pi: 0.0184
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1779-1820 bp	1.0	79.2
rps16-trnQ-UUG_p2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	1782-1823 bp	1.0	77.0
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	GGGTTTTGGTCCCGGTATTC	50.0	55.0	57.2	58.5	1800-1841 bp	1.0	75.5
rps16-trnQ-UUG_p4	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	1783-1824 bp	1.0	74.4
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.2	58.7	1809-1850 bp	1.0	74.3