Hotspot

ndhH

SSC · 124182-125363 · 1182 bp

Back to Myrospermum

Pi 0.479

MarkerSeek score 82.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 507
pi: 0.479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CCGGGATCCGTTGAATTTCA	TGTCTTAACGAATCGCACGT	50.0	45.0	58.3	57.9	2077 bp	0.5	57.5
ndhH_p2	GGGGGAGGGGCTTTAATTTT	TGTCTTAACGAATCGCACGT	50.0	45.0	57.7	57.9	2151 bp	0.5	57.5
ndhH_p3	CCGGGATCCGTTGAATTTCA	TCAGGTCCAATACGCTGTTG	50.0	50.0	58.3	57.9	1700 bp	0.5	57.2
ndhH_p4	GGGGGAGGGGCTTTAATTTT	TCAGGTCCAATACGCTGTTG	50.0	50.0	57.7	57.9	1774 bp	0.5	57.1
ndhH_p5	TTCTGGGAAAACGTCAACGA	TGTCTTAACGAATCGCACGT	45.0	45.0	57.7	57.9	1965 bp	0.5	57.1