Hotspot

ndhG

SSC · 128535-129065 · 531 bp

Back to Myrospermum

Pi 0.427

MarkerSeek score 80.20

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 198
pi: 0.427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGGTCAACAAACAGTCCGAG	GAAACAATAGCCGGTCCGAT	50.0	50.0	58.1	58.1	1690 bp	0.5	59.2
ndhG_p2	AGGCCGTTTACCAATGTCAG	GAAACAATAGCCGGTCCGAT	50.0	50.0	58.2	58.1	1297 bp	0.5	58.2
ndhG_p3	TGATTACCCTATCCCACGCA	GAAACAATAGCCGGTCCGAT	50.0	50.0	58.2	58.1	1643 bp	0.5	58.0
ndhG_p4	TGCTTTAGGCCGTTTACCAA	GAAACAATAGCCGGTCCGAT	45.0	50.0	57.7	58.1	1303 bp	0.5	57.4
ndhG_p5	TTGCTTTAGGCCGTTTACCA	GAAACAATAGCCGGTCCGAT	45.0	50.0	57.7	58.1	1304 bp	0.5	57.4