Hotspot

ndhD

SSC · 130276-131775 · 1500 bp

Back to Myrospermum

Pi 0.492

MarkerSeek score 82.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1500
variable_sites: 671
pi: 0.492
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAATTGCTTCTGCCCCAAGA	GCTAATGAGGCATGGGGATC	45.0	55.0	58.0	58.2	2293 bp	0.5	58.5
ndhD_p2	AATTGCTTCTGCCCCAAGAA	GCTAATGAGGCATGGGGATC	45.0	55.0	58.0	58.2	2292 bp	0.5	58.5
ndhD_p3	AAACAACTCGAAGCATGGGT	GCTAATGAGGCATGGGGATC	45.0	55.0	58.0	58.2	2191 bp	0.5	58.5
ndhD_p4	GGCATGAAACAACTCGAAGC	GCTAATGAGGCATGGGGATC	50.0	55.0	58.0	58.2	2197 bp	0.5	58.4
ndhD_p5	AATTGCTTCTGCCCCAAGAA	GGGAGCAGTATGGGCTAATG	45.0	55.0	58.0	57.8	2305 bp	0.5	58.4