Hotspot

rps8-rpl14

LSC · 88441-89177 · 737 bp

Back to Myrospermum

Pi 0.139

MarkerSeek score 83.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 91
pi: 0.139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TTATGTCAGCGATGGTGTCC	TTTCATTAGCACCCGAGGTA	50.0	45.0	57.7	56.2	777-791 bp	1.0	75.5
rps8-rpl14_p2	GTCAGCGATGGTGTCCTTAC	TTTCATTAGCACCCGAGGTA	55.0	45.0	58.4	56.2	773-787 bp	1.0	75.1
rps8-rpl14_p3	TATGTCAGCGATGGTGTCCT	TTTCATTAGCACCCGAGGTA	50.0	45.0	58.5	56.2	776-790 bp	1.0	73.9
rps8-rpl14_p4	TTATGTCAGCGATGGTGTCC	TCATTAGCACCCGAGGTATT	50.0	45.0	57.7	56.0	775-789 bp	1.0	73.6
rps8-rpl14_p5	TTATGTCAGCGATGGTGTCC	TTCATTAGCACCCGAGGTAT	50.0	45.0	57.7	56.0	776-790 bp	1.0	73.6