Hotspot

rps11-rpl36

LSC · 86616-87486 · 871 bp

Back to Myrospermum

Pi 0.154

MarkerSeek score 83.80

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 125
pi: 0.154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TCGTTGCATACCTTGATCCG	GAATGCGTGAGGGGGTTTAA	50.0	50.0	58.1	58.2	1593-1733 bp	1.0	88.9
rps11-rpl36_p2	ACTTCCACTGTAGTGTCCGA	GAATGCGTGAGGGGGTTTAA	50.0	50.0	58.0	58.2	1866-2005 bp	1.0	88.2
rps11-rpl36_p3	TTCCACTGTAGTGTCCGAGT	GAATGCGTGAGGGGGTTTAA	50.0	50.0	58.0	58.2	1864-2003 bp	1.0	88.2
rps11-rpl36_p4	GAAATCACCCGACCCAGTAC	GAATGCGTGAGGGGGTTTAA	55.0	50.0	58.0	58.2	1478 bp	0.5	58.5
rps11-rpl36_p5	AGAAATCACCCGACCCAGTA	GAATGCGTGAGGGGGTTTAA	50.0	50.0	58.1	58.2	1479 bp	0.5	58.2