Hotspot

psbZ-trnG-GCC

LSC · 40809-41408 · 600 bp

Back to Myrospermum

Pi 0.108

MarkerSeek score 84.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 600
variable_sites: 65
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	754-2450 bp	1.0	81.8
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	757-2453 bp	1.0	80.2
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	750-2446 bp	1.0	80.1
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	755-2451 bp	1.0	79.8
psbZ-trnG-GCC_p5	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	753-2449 bp	1.0	79.0